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panc1  (ATCC)
99
ATCC panc1
Effects of MAGEA1 knockdown on mRNA/protein expression and cellular functions including proliferation, viability, migration, and invasion. (A) Western blotting detected MAGEA1 expression in SK-OV-3 (ovarian cancer), <t>Panc1</t> (pancreatic cancer), HupT3 (pancreatic cancer), HeLa (cervical cancer), and T24 (urinary bladder carcinoma). GAPDH was used as a loading control. HeLa was transfected with shMAGEA1 #1 and #2 and shNC was established as a control. (B) Reverse transcription-quantitative PCR and (C) western blot analysis were used to estimate the efficiency of knockdown. (D) Cell viability was determined by MTT assay after 24 h of cell seeding. (E) A significant decrease in cell proliferation was observed in MAGEA1 knockdown compared with control. The number of proliferated cells is presented as a percentage of the control. (F) Representative images from wound scratch at different time points (magnification, ×40). (G) Percentages of wound closure at 24 and 72 h are shown as a bar graph. The scratched area and lines were quantified by ImageJ software with the MRI tool. (H) Cell migration and invasion were confirmed by Transwell migration and invasion assays. Representative images of cells are illustrated below. Scale bar, 500 μ m. (I) Quantification of migrated and invaded cells in distinct groups. The number of migrated and invaded cells is presented as a percentage of the control. Error bars represent the mean ± SEM. * P<0.05, ** P<0.01, *** P<0.005. MAGEA1, MAGE family member A1; sh, short hairpin; NC, negative control.
Panc1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/panc1/product/ATCC
Average 99 stars, based on 1 article reviews
panc1 - by Bioz Stars, 2026-04
99/100 stars
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human pancreatic cancer cell line panc 1  (ATCC)
99
ATCC human pancreatic cancer cell line panc 1
Effects of MAGEA1 knockdown on mRNA/protein expression and cellular functions including proliferation, viability, migration, and invasion. (A) Western blotting detected MAGEA1 expression in SK-OV-3 (ovarian cancer), <t>Panc1</t> (pancreatic cancer), HupT3 (pancreatic cancer), HeLa (cervical cancer), and T24 (urinary bladder carcinoma). GAPDH was used as a loading control. HeLa was transfected with shMAGEA1 #1 and #2 and shNC was established as a control. (B) Reverse transcription-quantitative PCR and (C) western blot analysis were used to estimate the efficiency of knockdown. (D) Cell viability was determined by MTT assay after 24 h of cell seeding. (E) A significant decrease in cell proliferation was observed in MAGEA1 knockdown compared with control. The number of proliferated cells is presented as a percentage of the control. (F) Representative images from wound scratch at different time points (magnification, ×40). (G) Percentages of wound closure at 24 and 72 h are shown as a bar graph. The scratched area and lines were quantified by ImageJ software with the MRI tool. (H) Cell migration and invasion were confirmed by Transwell migration and invasion assays. Representative images of cells are illustrated below. Scale bar, 500 μ m. (I) Quantification of migrated and invaded cells in distinct groups. The number of migrated and invaded cells is presented as a percentage of the control. Error bars represent the mean ± SEM. * P<0.05, ** P<0.01, *** P<0.005. MAGEA1, MAGE family member A1; sh, short hairpin; NC, negative control.
Human Pancreatic Cancer Cell Line Panc 1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human pancreatic cancer cell line panc 1/product/ATCC
Average 99 stars, based on 1 article reviews
human pancreatic cancer cell line panc 1 - by Bioz Stars, 2026-04
99/100 stars
  Buy from Supplier
panc 1  (ATCC)
99
ATCC panc 1
Effects of MAGEA1 knockdown on mRNA/protein expression and cellular functions including proliferation, viability, migration, and invasion. (A) Western blotting detected MAGEA1 expression in SK-OV-3 (ovarian cancer), <t>Panc1</t> (pancreatic cancer), HupT3 (pancreatic cancer), HeLa (cervical cancer), and T24 (urinary bladder carcinoma). GAPDH was used as a loading control. HeLa was transfected with shMAGEA1 #1 and #2 and shNC was established as a control. (B) Reverse transcription-quantitative PCR and (C) western blot analysis were used to estimate the efficiency of knockdown. (D) Cell viability was determined by MTT assay after 24 h of cell seeding. (E) A significant decrease in cell proliferation was observed in MAGEA1 knockdown compared with control. The number of proliferated cells is presented as a percentage of the control. (F) Representative images from wound scratch at different time points (magnification, ×40). (G) Percentages of wound closure at 24 and 72 h are shown as a bar graph. The scratched area and lines were quantified by ImageJ software with the MRI tool. (H) Cell migration and invasion were confirmed by Transwell migration and invasion assays. Representative images of cells are illustrated below. Scale bar, 500 μ m. (I) Quantification of migrated and invaded cells in distinct groups. The number of migrated and invaded cells is presented as a percentage of the control. Error bars represent the mean ± SEM. * P<0.05, ** P<0.01, *** P<0.005. MAGEA1, MAGE family member A1; sh, short hairpin; NC, negative control.
Panc 1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/panc 1/product/ATCC
Average 99 stars, based on 1 article reviews
panc 1 - by Bioz Stars, 2026-04
99/100 stars
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human panc1 cell line  (ATCC)
99
ATCC human panc1 cell line
Effects of MAGEA1 knockdown on mRNA/protein expression and cellular functions including proliferation, viability, migration, and invasion. (A) Western blotting detected MAGEA1 expression in SK-OV-3 (ovarian cancer), <t>Panc1</t> (pancreatic cancer), HupT3 (pancreatic cancer), HeLa (cervical cancer), and T24 (urinary bladder carcinoma). GAPDH was used as a loading control. HeLa was transfected with shMAGEA1 #1 and #2 and shNC was established as a control. (B) Reverse transcription-quantitative PCR and (C) western blot analysis were used to estimate the efficiency of knockdown. (D) Cell viability was determined by MTT assay after 24 h of cell seeding. (E) A significant decrease in cell proliferation was observed in MAGEA1 knockdown compared with control. The number of proliferated cells is presented as a percentage of the control. (F) Representative images from wound scratch at different time points (magnification, ×40). (G) Percentages of wound closure at 24 and 72 h are shown as a bar graph. The scratched area and lines were quantified by ImageJ software with the MRI tool. (H) Cell migration and invasion were confirmed by Transwell migration and invasion assays. Representative images of cells are illustrated below. Scale bar, 500 μ m. (I) Quantification of migrated and invaded cells in distinct groups. The number of migrated and invaded cells is presented as a percentage of the control. Error bars represent the mean ± SEM. * P<0.05, ** P<0.01, *** P<0.005. MAGEA1, MAGE family member A1; sh, short hairpin; NC, negative control.
Human Panc1 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human panc1 cell line/product/ATCC
Average 99 stars, based on 1 article reviews
human panc1 cell line - by Bioz Stars, 2026-04
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crl  (ATCC)
99
ATCC crl
Effects of MAGEA1 knockdown on mRNA/protein expression and cellular functions including proliferation, viability, migration, and invasion. (A) Western blotting detected MAGEA1 expression in SK-OV-3 (ovarian cancer), <t>Panc1</t> (pancreatic cancer), HupT3 (pancreatic cancer), HeLa (cervical cancer), and T24 (urinary bladder carcinoma). GAPDH was used as a loading control. HeLa was transfected with shMAGEA1 #1 and #2 and shNC was established as a control. (B) Reverse transcription-quantitative PCR and (C) western blot analysis were used to estimate the efficiency of knockdown. (D) Cell viability was determined by MTT assay after 24 h of cell seeding. (E) A significant decrease in cell proliferation was observed in MAGEA1 knockdown compared with control. The number of proliferated cells is presented as a percentage of the control. (F) Representative images from wound scratch at different time points (magnification, ×40). (G) Percentages of wound closure at 24 and 72 h are shown as a bar graph. The scratched area and lines were quantified by ImageJ software with the MRI tool. (H) Cell migration and invasion were confirmed by Transwell migration and invasion assays. Representative images of cells are illustrated below. Scale bar, 500 μ m. (I) Quantification of migrated and invaded cells in distinct groups. The number of migrated and invaded cells is presented as a percentage of the control. Error bars represent the mean ± SEM. * P<0.05, ** P<0.01, *** P<0.005. MAGEA1, MAGE family member A1; sh, short hairpin; NC, negative control.
Crl, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crl/product/ATCC
Average 99 stars, based on 1 article reviews
crl - by Bioz Stars, 2026-04
99/100 stars
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panc 1 atcc  (ATCC)
99
ATCC panc 1 atcc
Effects of MAGEA1 knockdown on mRNA/protein expression and cellular functions including proliferation, viability, migration, and invasion. (A) Western blotting detected MAGEA1 expression in SK-OV-3 (ovarian cancer), <t>Panc1</t> (pancreatic cancer), HupT3 (pancreatic cancer), HeLa (cervical cancer), and T24 (urinary bladder carcinoma). GAPDH was used as a loading control. HeLa was transfected with shMAGEA1 #1 and #2 and shNC was established as a control. (B) Reverse transcription-quantitative PCR and (C) western blot analysis were used to estimate the efficiency of knockdown. (D) Cell viability was determined by MTT assay after 24 h of cell seeding. (E) A significant decrease in cell proliferation was observed in MAGEA1 knockdown compared with control. The number of proliferated cells is presented as a percentage of the control. (F) Representative images from wound scratch at different time points (magnification, ×40). (G) Percentages of wound closure at 24 and 72 h are shown as a bar graph. The scratched area and lines were quantified by ImageJ software with the MRI tool. (H) Cell migration and invasion were confirmed by Transwell migration and invasion assays. Representative images of cells are illustrated below. Scale bar, 500 μ m. (I) Quantification of migrated and invaded cells in distinct groups. The number of migrated and invaded cells is presented as a percentage of the control. Error bars represent the mean ± SEM. * P<0.05, ** P<0.01, *** P<0.005. MAGEA1, MAGE family member A1; sh, short hairpin; NC, negative control.
Panc 1 Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/panc 1 atcc/product/ATCC
Average 99 stars, based on 1 article reviews
panc 1 atcc - by Bioz Stars, 2026-04
99/100 stars
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Effects of MAGEA1 knockdown on mRNA/protein expression and cellular functions including proliferation, viability, migration, and invasion. (A) Western blotting detected MAGEA1 expression in SK-OV-3 (ovarian cancer), Panc1 (pancreatic cancer), HupT3 (pancreatic cancer), HeLa (cervical cancer), and T24 (urinary bladder carcinoma). GAPDH was used as a loading control. HeLa was transfected with shMAGEA1 #1 and #2 and shNC was established as a control. (B) Reverse transcription-quantitative PCR and (C) western blot analysis were used to estimate the efficiency of knockdown. (D) Cell viability was determined by MTT assay after 24 h of cell seeding. (E) A significant decrease in cell proliferation was observed in MAGEA1 knockdown compared with control. The number of proliferated cells is presented as a percentage of the control. (F) Representative images from wound scratch at different time points (magnification, ×40). (G) Percentages of wound closure at 24 and 72 h are shown as a bar graph. The scratched area and lines were quantified by ImageJ software with the MRI tool. (H) Cell migration and invasion were confirmed by Transwell migration and invasion assays. Representative images of cells are illustrated below. Scale bar, 500 μ m. (I) Quantification of migrated and invaded cells in distinct groups. The number of migrated and invaded cells is presented as a percentage of the control. Error bars represent the mean ± SEM. * P<0.05, ** P<0.01, *** P<0.005. MAGEA1, MAGE family member A1; sh, short hairpin; NC, negative control.

Journal: International Journal of Oncology

Article Title: Therapeutic implications of targeting cancer testis antigen MAGEA1 in cervical cancer

doi: 10.3892/ijo.2026.5870

Figure Lengend Snippet: Effects of MAGEA1 knockdown on mRNA/protein expression and cellular functions including proliferation, viability, migration, and invasion. (A) Western blotting detected MAGEA1 expression in SK-OV-3 (ovarian cancer), Panc1 (pancreatic cancer), HupT3 (pancreatic cancer), HeLa (cervical cancer), and T24 (urinary bladder carcinoma). GAPDH was used as a loading control. HeLa was transfected with shMAGEA1 #1 and #2 and shNC was established as a control. (B) Reverse transcription-quantitative PCR and (C) western blot analysis were used to estimate the efficiency of knockdown. (D) Cell viability was determined by MTT assay after 24 h of cell seeding. (E) A significant decrease in cell proliferation was observed in MAGEA1 knockdown compared with control. The number of proliferated cells is presented as a percentage of the control. (F) Representative images from wound scratch at different time points (magnification, ×40). (G) Percentages of wound closure at 24 and 72 h are shown as a bar graph. The scratched area and lines were quantified by ImageJ software with the MRI tool. (H) Cell migration and invasion were confirmed by Transwell migration and invasion assays. Representative images of cells are illustrated below. Scale bar, 500 μ m. (I) Quantification of migrated and invaded cells in distinct groups. The number of migrated and invaded cells is presented as a percentage of the control. Error bars represent the mean ± SEM. * P<0.05, ** P<0.01, *** P<0.005. MAGEA1, MAGE family member A1; sh, short hairpin; NC, negative control.

Article Snippet: HeLa (CCL-2) and Panc1 (CRL-1469) were purchased from American Type Culture Collection.

Techniques: Knockdown, Expressing, Migration, Western Blot, Control, Transfection, Reverse Transcription, Real-time Polymerase Chain Reaction, MTT Assay, Software, Negative Control